A CRISPR/Cas12a-MXene Nanozyme Platform for Universal Detection of Trace DNA

The Quick Summary

We created a new, simple way to find tiny amounts of specific DNA without needing big lab machines. This system uses a smart gene-editing tool called CRISPR/Cas12a combined with tiny MXene particles that can change color. When the target DNA is found, the particles react to show a visible color change, making detection easy and fast.

Practical Implications

This study provides a practical, instrument-free method for quickly detecting specific DNA markers in food, which could significantly improve food safety by enabling rapid screening for pathogens or adulterants on-site.

Potential Use in Indonesia

In Indonesia, this technology could be invaluable for ensuring the safety of street food and fresh produce sold in traditional markets, where access to advanced laboratories is often limited. Vendors could perform simple, visual checks for common foodborne pathogens, mitigating health risks exacerbated by tropical climates and rapid spoilage, and strengthening local food supply chains.

Original Abstract

The instrument-free detection of specific DNA sequences is critical for point-of-care diagnostics yet remains challenging. To address this, we developed a universal colorimetric biosensing platform integrating the CRISPR/Cas12a system with MXene nanozymes. Target recognition by the Cas12a/crRNA complex triggers trans-cleavage of single-stranded DNA inhibitors, restoring the peroxidase-like activity of MXenes to produce a visible signal. The platform achieved a detection limit of 132 copies/μL……

Read the full peer-reviewed publication here